Sentence examples for transformants obtained by from inspiring English sources
As result, 40 mM EDTA treatment increased the number of transformants obtained by eightfold in comparison to the conventional protocol using plasmid DNA.
Transformants obtained by allelic exchange mutagenesis of H. pylori 26695/arsS::km were checked for the correct integration of the mutated arsS alleles by PCR analysis with primer pairs flanking the integration site.
In this study, T-DNA insertion loci were recovered from L. maculans transformants obtained by ATMT for which germinating conidia (incubated for 48 hr) were used (Blaise et al. 2007).
On average, the transformant obtained by random integration displayed lower glucoamylase activity than the transformant obtained by targeted integration and could be due to the targeting of the expression cassette to the cbh2 locus, which is likely to be an actively transcribed region with a positive effect on glucoamylase production which is driven by the cbh1 promoter.
Complementation assays to confirm the putative interactions in a clean yeast background were done by transforming bait and prey plasmids into AH109 and Y187 yeast strains respectively, and mating the transformants obtained (identified by their ability to grow on tryptophan- or leucine-deficient SD medium).
3G86.1 and 3G86.32 come from the same initial clone that turned out to be a double transformant; single transformants were obtained by plasmid extraction and retransformation.
The presence of the respective construct in the transformants obtained was confirmed by PCR on DNA extracted from the different colonies using specific oligonucleotides for the specific inserts.
The putative transformants obtained were analyzed by the phosphinothricin leaf paint assay (Fig. 2F).
Using double markers enabled the transformants obtained on pyrEF selection to be verified by a simple X-gal (5-bromo-4-chloro-3-indolyl-β-D galactopyranoside) screening.
On the 123 primary transformants obtained, 18 were homozygous for the mutation (nof1-1) asuggesteded by the resistance of their progenies to kanamycin and confirmed by genotyping by PCR (data not shown).
pSP-GM1 was linearized with restriction enzyme BglII (ThermoFisher), purified, and co-transformed together with purified xfpk PCR-fusion constructs into CEN.PK 113-5D, and transformants were obtained by growth on SD-URA plates.
