Sentence examples for mutants were labeled from inspiring English sources

Briefly, R mutants were labeled using the serum of a B. ovis infected sheep and a Texas Red conjugated monoclonal antibody to sheep IgG and the parental strains using the serum from a B. abortus infected cattle and an Alexa® 594 (Molecular Probes; Eugene, OR) conjugated anti-cattle monoclonal antibody.

Cysteine mutants were labeled, reconstituted in POPC/POPG liposomes, and subjected to CW-EPR measurements at 0 mV.

Cells expressing the tagged IR mutants were labeled using ACP-S which transfers a 4'-phosphopantetheine group from the CoA to the A1 sequence (in red, Figure  1A).

The double mutant, S143C/S336C, as well as the corresponding single-cysteine mutants, were labeled with the MTSL spin label (see Experimental Procedures) to attach the R1 side chain.

Purified MscL mutants were labeled with Alexa Fluor 488 (and88) Alexalexa Fluor 568 (and68) and reconstituted into ∼50 nm liposomes made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with 2% 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-biotinyl (BPE).

Wt and nif mutants were labelled with pMC1-EPS-RtGFP and inoculated to root zones of 12 day-old seedlings derived from surface-sterilized.

To study how disrupting the SapM locus and creating a deficiency in ManLAM capping affects the efficiency of binding and phagocytosis of the mycobacteria, M. bovis BCG WT and mutants were labelled with fluorescein isothiocyanate (FITC) and their uptake by murine Mf4/4 macrophages was analysed by flow cytometry.

Four mutants that are predicted to have reduced splicing were designed not to have any structures with contact conformation or otherwise short structural branchpoint distances (these mutants are labeled with letter 'L', which stands for long d s ).

Four mutants that are predicted to splice efficiently were designed to have multiple suboptimal structures with contact conformation and short average structural branchpoint distance (these mutants are labeled with letter 'S', which stands for short d s ).

Recombinant CR2 and K41E CR2 mutant were labeled at 1 mg/mL by incubating [99mTc(CO)3(OH2)3]+ with 100 µg of protein at 37°C with gentle shaking for up to 120 min. Radiochemical yield was determined by ITLC (Figure 5a).

The purified cDNAs prepared from B. pseudomallei wild type or σE mutant were labeled with Cy5 or Cy3 respectively (Cy5-ULS Cy3-ULS, Kreatech Diagnostics).