Vascular bundle organisation defects in pxy mutants are characterised by intercalation of xylem and phloem, which is in contrast to the wild type where they are spatially separated.
It is located on scaffold 2: 786955-789433 (ID 120117), and the mutant is characterised by a loss of a 2478-base pair fragment, which starts downstream of the region encoding the CRE1 zinc finger and reaches into the 3'-non-coding region [ 54].
Four mutant enzymes were characterised, pooled and subjected to DNA shuffling [64].
The prostate lesions found in heterozygous Men1 mutant mice were characterised by a slow cancer development process, which ranged from in situ carcinoma to invasive adenocarcinoma.
Mice for each mutant allele were characterised using a standardised battery of clinical phenotyping procedures, as described previously (White et al., 2013) and summarised in Fig. S2.
Two lines obtained from T-DNA mutant collections were characterised by sequencing from both T-DNA borders: SALK_102345, an insertion in the last exon of REV (At5g60690) upstream of the termination codon, and GABI-KAT line GK_302G09, an insertion affecting the second exon of CUC3 (At1g76420).
Insertion events were characterised using Southern blot analysis and three of the Disease Attenuated Fusarium mutants, called DAF139, DAF140 and DAF141 were chosen for analysis in this study.
BMDCs were characterised by FACS.
Ideally, each mutant should be characterised to define the most likely effect it had on TetA(B) folding and activity, thus identifying those mutants most likely to improve the probability of obtaining well-diffracting crystals.
