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Pine genomic DNA and chloroplast clones were labeled using the Megaprime DNA Labeling System (GE Healthcare).
These clones were labeled with either Spectrum Orange (Vysis, Inc).
Clones were labeled in different colors (RFP, GFP) in the same animal using twin-spot MARCM.
The RRM1 and RRM2B targeted BAC clones were labeled with Texas Red fluorochrome by Nick translation.
DNA inserts from 10 clones were labeled with [P]dCTP using the Random Primer Labeling Method (Invitrogen).
(F ) Control (MARCM driver) and bap55 LL5955 type I MARCM clones were labeled with Dpn, Ase and CD8.
Similar(46)
BAC clones were labelled with digoxigenin or fluorescein-12-dUTP and Nick translation mix (Roche).
Candidate clones were labelled with a second chromophore rhodamine (tetrametyl rhodamine 5'dUTP)(Roche, Germany) in a similar manner.
The clones were labelled by triple immunofluorescence for the expression of cytokeratins 18 and 19 (predominantly luminal cells in vivo) and cytokeratin 14 (predominantly myoepithelial cells in vivo).
Microsatellite containing clones were labelled with digoxigenated dUTP (Dig-11dUTP) using the random priming method and in situ hybridized to polytene chromosomes according to [ 64].
PCR amplified inserts of clones were labelled with biotin 16-dUTP (Roche) or digoxigenin 11-dUTP (Roche) by random priming (Bioprime & Random primer kit; Invitrogen).
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