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Mutants were generated using the Takara MutanBEST Kit.
K3036.58E MOP receptor and E2976.58K KOP receptor mutants were generated.
Lysine (K) to Arg (R) point mutants were generated using site-directed mutagenesis.
HLA-A2 mutants were generated using overlap extension polymerase chain reaction.
shRNA-resistant TFEB mutants were generated using site-directed mutagenesis (Q5 0552S, New England Biolab).
The ttr-11 km64 ttr-11 km64(km85) deletion mutands ttr-57 km85 ttr-57 km85e CRISPR-Cas9 system41.
Five affinity mutants were generated covering binding constants between 400 nM and 11.7 mM.
FANCM wild-type and indicated mutants were generated by PCR and subcloned into NBLV0051 (Novo Bio) vector containing a 3× N-terminal Flag-tag.
SPOP Y87C/F133V/W131G mutants were generated by QuickChange II site-directed mutagenesis kit (Aligent Technology) with MYC-SPOP WT as a template.
This means that all the possible mutants were generated.
Point mutants were generated by Site-Directed Mutagenesis kit (NEB) according to the manufacturer's instructions.
More suggestions(15)
clones were generated
mutants were named
mutants were indicated
mutants were affected
mutants were expressed
mutants were designed
mutants were screened
mutants were solved
mutants were compared
mutants were confirmed
mutants were obtained
mutants were recovered
mutants were discovered
mutants were set
mutants were monitored
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