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Generally, clustered and nonclustered mutants were affected similarly.
These mutants were affected in pore formation, toxin oligomerization and receptor binding, respectively.
The oligomeric structure of wild type Cry1Ab toxin was mainly found inserted into the membrane pellet, in contrast with the helix α-4 mutants, that remained in the soluble fraction suggesting that helix α-4 mutants were affected in membrane insertion (Fig. 2A).
Experiments were performed to establish if the three mutants were affected by an impairment of the NH2-COOH terminal interaction, and if so, if this impairment would be amenable to normalization using conditions that enhance stability of binding, for instance by increasing concentrations of natural or synthetic AR agonists.
As indicated by the values of their specific activities, all the mutants were affected in the PZase activity, with no detectable PZA hydrolysis for Cys138Ala, Lys96Gln and Asp49Gly, and a very low residual activity for Ala134Val, His51Ala, His57Asp, Trp68Leu and Phe13Leu (Table 2).
All mutants were affected in oil remobilization.
Similar(43)
Fig. 3 docs mutants are affected in gravitropic responses.
Phenotypic characterization shows that all five mutants are affected to varying extents in their ability to synthesize late viral proteins at the nonpermissive temperature, similar to other ts mutants with lesions in the 22- and the 147-kDa subunits of the VV RNA polymerase.
The nof1 mutants are affected in female gametogenesis and embryo development.
Several of the irx mutants are affected in genes encoding Type II membrane GTs that appear to be involved in xylan biosynthesis.
In agreement with this function, we showed that the nof1 mutants are affected in the methylation of rDNA promoter and rRNA expression.
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