The maximum reporter response for each mutant was set to 100%.
Simulations in which the trimerization rate constant (k tri ) for the F408V mutant was set to one-tenth its best value for the wild-type simulation generated a good fit to the observed hemifusion times, when all other parameters were held constant.
Similarly, the mtDNA/nDNA ratio of the untreated MTS-hOGG1 and MTS-mutant hogg1 were set at 100% for calculating values obtained with recombinant wild-type (hOGG1) or mutant (hogg1) respectively.
Reactions to detect wild-type and mutant alleles were set up in parallel for the same genomic DNA: 1 cycle of 94°C 5 min, 35 cycles of 94°C 30 s, 62°C 30 s for the wild-type allele or 67°C for the null allele, 72°C 60 s and 1 cycle of 72°C 5 min. Addition of 1% DMSO was required for the null allele genotyping.
(C ) Binding reactions with the RA104 mutant transposase were set up as in Figure 3B.
The same thresholds for both control and mutant embryos were set up to reflect the expression of mCherry throughout the whole embryo.
This was confirmed for Sod n64/ Sod n1 trans-heterozygous mutants where crosses were set up in the opposite direction.
The features of the WT and mutant amino acids were set to 1 or −1, respectively, and the rest of the features were set to 0. the side chain accessible surface area [calculated by NACCESS (Hubbard et al., 1991)] was averaged over all the protein structures of the query protein (F7).
To explore the key underlying molecular switches resulting in the male sterility of the cotton GMS mutant, six DGE libraries were set up during the anther development of Dong A WT and GMS mutant in the current work.
To generate the mutant collection, mutagenesis conditions were set to induce 90%% cell mortality, ensuring a high mutation frequency.
