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Exact(9)
Mutants were genotyped by PCR as described previously for Pafah1b1 [11], Reelin [42], Apoer2 and Vldlr [9], and Dab1 [6].
Double mutants were genotyped using PCR.
Mutants were genotyped using primers shown in Additional file 4: Table S3.
For subsequent experiments, tmod4 trg mutants were genotyped by using the same protocol.
The T-DNA insertional mutants were genotyped by using a PCR-based method (Alonso et al., 2003).
Tsc1 mutants were genotyped by tail-derived genomic DNA PCR as described previously (Uhlmann et al., 2002b).
Similar(51)
Homozygous Atlyk5-2 mutand and Atlyk4/Atlyk5-2 double mutants plants were genotyped using primers listed in Supplemental file 1.
The homozygous wild-type, heterozygous, and homozygous mutant offspring produced from the intercrosses of heterozygous mutant mice were genotyped either by TGCE or DNA sequencing of PCR products.
Adult PTBP1 mutant mice were genotyped from a tail clip after proteinase K digestion using 2 primer pairs.
The trpm mutant larvae were genotyped and separated in advance using a CyO balancer chromosome tagged with Actin5C-eGFP transgene (stock 1, above) [25].
Wt and Uch37 mutant embryos were genotyped using P (5'-TTA ATG TGA ATA ATC GGA ATG CTG G-3') and Q (5'-CTA TAC AAT ATG GTA TCT GAT TTG G-3') to detect the Wt gene.
Related(20)
clones were genotyped
genes were genotyped
transformants were genotyped
variants were genotyped
mutants were kept
mutants were confirmed
mutants were obtained
mutants were recovered
mutants were validated
mutants were discovered
mutants were generated
mutants were set
mutants were indicated
mutants were named
mutants were affected
mutants were expressed
mutants were designed
mutants were solved
mutants were screened
mutants were compared
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