Sentence examples for transformants were genotyped from inspiring English sources
The transformants were genotyped for the presence of the ire1Δ::KANMX cassette and the absence of IRE1+, to obtain strains SCMSP176 (ire1Δ::KANMX in the X2180-5B background), SCMSP207 (ire1Δ::KANMX in the CEN.PK background), and SCMSP210 (ire1Δ::KANMX in the YPH363 background).
Diploids, heterokaryons and homokaryotic mini-colonies (hypBΔ) or non-sporulating colonies (hypBΔ geaA1) of transformants were genotyped by PCR (primers 14 and 19) and/or by Southern blots.
Transformants were selected by spraying progeny with a 0.578% solution of Finale (1% glufosinate-ammonium, Bayer CropScience http://www.bayercropscience.com/); resistant T1 plants were genotyped for the fln1-1 insertion and 14 heterozygotes (independent transformation events) carried forward to the T2 generation.
Transformants were screened for their proper genotype by selecting for kanamycin resistance and colony PCR (GoTaq Green polymerase, Promega) using primers that flank the target gene.
The genotypes of transformants were confirmed by PCR and Southern blotting, as described previously (Barelle et al., 2004).
The genotypes of BCG transformants were verified by Southern blotting: two of the tested recombinant strains were of the desired genotype carrying a single copy of the plasmid at the attB site in the bacterial chromosome (Fig. S2).
Afterwards, single spore isolates of the primary transformants were screened for homokaryotic ∆Smmob3:: hyg/∆ku70:: nat genotype on hygromycin B and nourseothricin containing BMM medium.
Transformants were isolated, and gene insertion and segregation confirmed by genotyping (data not shown).
Thirty-one E12-Ω-CaMV-35S E12-Ω-CaMV-35S E12-Ω-CaMV-35S E12-Ω-CaMV-35Sh on selecTcBBMand subsequentransformantsing.
Transformants were selected on YNBUra YNBLeu or YNB depending on their genotype.
T1 seeds were sown on hygromycin plates and positive transformants were transferred to soil followed by subsequent verification of the genotype using PCR.
