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The other conditions for enzyme production/optimization with mutants were kept constant.
For storage, mutants were kept on TAP agar plates in dim light.
Ultimately, 33 mutants were kept and frozen in glycerol stocks (Table 1).
All crosses with the dia 5 mutants were kept at 25oC as this allele is temperature sensitive (Homem and Peifer, 2009).
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Gli2lacKI, Gli3Xt-J and Sufu mutants are kept on a 129S2/SvPasCrl background and genotyped as reported [17], [30], [31].
Leaves of the Arabidopsis mes16 mutant were kept in the dark for 4 days.
As such, when a mutant allele at some locus fixed in the population, the frequency of the mutant was kept at fixation, removing the possibility of back mutations, for 3200 generations, the mutant frequency then being set to zero.
Df 2L dLRCH mutant flies were kept and selected using GFP-expressing CyO balancers.
Mutant chromosomes were kept over TM6c Tb, Sb balancer chromosomes.
The was mutant fish were kept in E3 supplemented with propylthiouracil (from 24 hpf until fixation) to suppress pigmentation.
However, when cyp709b3 mutant seedlings were kept on salt plates, more seedlings became bleached and dead.
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