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The resultant mutants were validated by a PCR approach using the respective primers (Table 2).
Putative mutants were validated by PCR amplification and sequencing of genomic DNA flanking the deletion.
Mutants were validated by whole-cell PCR with primers 5′ and 3′ of the cdc5 gene.
Candidate rosette defect mutants were validated through repeated rounds of limiting dilution prior to re-screening in ACM.
These mutants were validated by comparing their ability to stimulate PolB1, Fen1 or Lig1 individually on their respective optimal model substrates.
The results of the novel mutants were validated by comparative analyses with the already characterized mutants R116W [ 15, 18], R167W [ 19– 21] and R173W [ 21– 21].
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The production of macedocin under hyperosmotic conditions solely by the osmr mutants was validated by the well diffusion assay and by mass spectrometry analysis.
Lack of dmGluRA in drep-2 ex13 ; dmGluRA 112b double mutants was validated by single-fly PCR.
Several hygromycin resistant colonies were examined, and the Δ cld mutant was validated by PCR analysis.
e The Mosdi1 ReGFP mutants (M1 M9) were validated by dialogistic PCR.
f The Mosdi1 ReGFP mutants (M1 M9) were validated by southern blot.
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ones were validated
shifts were validated
mutants was validated
mutants were named
mutants were affected
mutants were indicated
mutants were expressed
mutants were designed
mutants were confirmed
mutants were kept
mutants were recovered
mutants were obtained
mutants were discovered
mutants were generated
mutants were set
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