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Consequently, the G126S and R133L mutants show higher catalytic efficiency as compared with the wild type.
The mutants show higher affinity and coupling efficiency for chrysene with faster rates of product formation compared to the wild type.
Interestingly, both mutants show higher amounts (factor ∼2) of mitochondrial HSP60 (Fig. 6A) which has been shown to be an important factor for the proper folding of proteins imported into mitochondria of the nematode Caenorhabditis elegans [42].
Several neuroendocrine pathway mutants and eating defective mutants show higher or lower fat storage levels than wild type, however, these mutants still show differences in fat stores when grown on different bacterial strains.
bar mutants show higher levels of S-nitrosylated proteins in heart and blood vessel cells as well as in pronephros as measured by an antibody against S-nitroso-cysteine (SNO-Cys).
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Resetting studies revealed that Rev-Erbα−/−Per1Brdm1 double mutants show high amplitude resetting (Fig. 3A F).
Interestingly, both pmt1 and pmt4 mutants show high temperature sensitivity at 39°C, which may have a great impact on infectivity in the mouse model.
Genes deregulated in the rx3 -/- mutants show high levels of connectivity to each other.
Among the candidate mutants, the A8T, A92E, N97Q and T245S mutants showed higher organic solvent stability at various methanol concentrations.
With the addition of 3% MnCl2, all the mutants showed higher ethanol production from 14.11 to 15.72 g/L compared to the R-control which produced 9.8 g/L, and the other used mutants with 1, 10 and 20% concentrations of MnCl2.
The SCCmec-deleted mutants showed higher colony spreading than their respective parent strains (Figure 1C and 1D).
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