Measurement of the percentage of metabolically inactive cells, unable to efflux fluorescent dyes such as phloxine B (Figure 4B) or propidium iodide (Figure 4C), yielded similar results: short after entering stationary phase, the mitochondrial mutants display high percentages of metabolically inactive cells, when compared to wild-type cultures.
Upon transient Mn depletion, smf-1 eh5) smf-1 eh5(ok1035) mutands smf-3 ok1035gher smf-3 ok1035ut they underwent a gradual Fe depletion with increasing mutantss (Fig. 5B).
The Δ tul1 and Δ sah2 mutants displayed higher secreted protein levels and higher enzyme activity during a 7-day time course as compared to the QM6a parental strain (Fig. 8).
In addition, trol mutants displayed higher levels of Hedgehog (Hh), morphogen, nearer to its source of expression, suggesting that Trol is required for diffusion of Hh (Park et al. 2003).
All the four mutants displayed high levels of sensitivity to the DNA damaging agents (Figs. 3B, 3C), compared to the wild-type, as reported earlier for the transposon induced recD mutant (CS1) of P. syringae [6].
In addition triple mutants displayed high levels of embryonic and larval lethality, with synthetic lethality being even more prominent in bro-1 rnt-1; unc-62; ceh-20 RNAi) ceh-20 RNAipplementanimalserial Fig. supplementary
This mutant displayed higher seed oil and lower seed protein levels compared to the wild-type background.
In the stems of the RG mutant, where lower insoluble lignin was quantified, we observed that the RG mutant displayed higher saccharification efficiency than WT.
This mutant displayed higher rates of apoptosis in cortical Tgfβ1−/− neurons, which was considered to be the main cause for loss of neurons (Brionne et al. 2003).
Since the DrAqp3b-H53A/G54H mutant displayed higher water permeability at pH 6 than the WT, and the DrAqp3b-T85A was more permeable and not affected by alkaline pH, we finally designed and characterized a triple mutant DrAqp3b-H53A/G54H/T85A (Table 2).
