By contrast, both d3 and d10 mutants contained higher N in their leaves under N-deficient conditions.
The sultr4;1/sultr4;2 double knock-out mutants contained higher amounts of sulphate than did wild-type plants.
As observed with bri1-301, the cp3 mutant contained higher levels of CPD transcripts compared to the wild type Ler in the presence of BL, indicating that the cp3 mutant possesses reduced-BL sensitivity.
The bas1-2 sob7-1 double mutant contains higher BR levels and flowers earlier than the wild type, demonstrating a role for these BR-inactivating enzymes in floral induction (Turk et al. 2005).
FRA3, a type II 5PTase gene, plays an essential role in secondary wall synthesis in fibre cells and xylem vessels, and the fra3-knockout mutant contains higher amounts of PtdIns(4,5) P2 and Ins(1,4,5) P3 and shows reduced thickness of the secondary wall and decreased stem strength owing to the disruption in actin organization [ 41].
The orange/red fruit (Delta mutant) contains high levels of δ-carotene.
The triple mutants did contain higher C15iso and C17iso levels than fat-6;fat-7, but only a slight reduction in 18∶0 (Figure S2B and data not shown).
Among the 31 validated mutants, 5 contained higher oil content than wild-type under nitrogen-replete conditions (type I), 13 had altered oil content under nitrogen-depleted conditions (type II), and all 31 mutants showed changed capacity in oil remobilization (type III).
The M. oryzae Δ abc3 mutants also contained higher endogenous levels of H2O2, and if this function can be proven also for C. rosea B-III ABC transporters it is interesting to speculate about a connection between the expansions of ABC B-III genes and the AA3_2 subfamily genes encoding H2O2-producing enzymes.
We also examined two other mutants reported to contain high fat stores.
