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Oxygen consumption, and therefore respiration, was severely inhibited in the mutants, as expected (Figure 3A).

The SPIK AtAPY2 construct was always present in the DKO-GFP mutants as expected, but interestingly also in the DKO-SNAP mutants.

In addition, Foxg1 mRNA was also reduced in these mutants as expected for heterozygote Foxg1-cre mice (42) (Supplementary Material, Fig. S2Cg).

Considering the single mutants, the ratio between C16:1 and C16:0 was decreased for the fat-5 mutant, as expected due to the fact that the palmitoyl-CoA desaturase has been knocked out; the ratio between C18 1n9 and C18 0 decreased for both fat-6 and fat-7 mutants, as expected for the reduction in expression of stearoyl-CoA desaturase.

The COH-3/4 antibody recognizes COH-3 and COH-4 specifically, since staining was undetectable in coh-4 coh-3 double mutants, but strong staining persisted in both single mutants, as expected from the complete genetic redundancy of the coh-4 and coh-3 genes (Severson et al., 2009).

Similar(54)

By contrast, the cbbLS::kanr mutant, as expected, did not express any L8S8 RubisCO species (Figure 5C).

The CPY* control is degraded rapidly in wild type cells and stabilized in the Δcue1 ERAD mutant as expected (Figure 6, upper left).

Moreover, repression of fimA, ihfA, and ybaJ by Hha was further corroborated by quantitative real time PCR (RT-PCR) using RNA from 15 h LB glu biofilm cells since we found fimA, ihfA, and ybaJ were induced in the hha mutant as expected: fimA 6.1±0.2-fold, ihfA 3.1±0.1-fold, and ybaJ 8.0±0.5-fold.

However, 1-isochromanone was detected following bioconversion of 1-indanone by cells expressing the M446G mutant as expected.

In the Tgif homozygous mutant, as expected there are no epithelial cells positive for TGIF and significantly fewer cells positive for pSmad2.

Molecularly, the serrated polyps were significantly more methylated and BRAF mutant as expected, and all polyp subtypes had a low KRAS mutation rate (Table  3).

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