We observed significant differences in the ultimate fate of carbon in the zwf mutant as predicted with the FBA, MOMA and PSEUDO objective functions.
Data are shown in Figure 4. We first confirmed that binding of 70 nM [H]-AR-C155858 was totally blocked in the SG/FY mutant as predicted from the transport studies.
The results are as predicted.
The line-up is roughly as predicted.
Thus the pattern of starch degradation in these mutants is as predicted from the models for a situation in which the above factor is incorrectly set to a value <1.
The catalytic activity of the E281K mutant was severely diminished, as predicted by the results of tetramerisation assays and 3(c)).
As predicted, mutant ITCH tested in parallel gave only a baseline signal at all concentrations tested, further confirming that the signal detected in our experimental conditions was dependent on the Ub ligase activity of ITCH.
As predicted, the mutants exhibited decreased thermal stability, increased sensitivity to NADH and the binding of E3Y18F to the E3-depleted core of the pig heart PDC increased E3 activity and decreased E3 sensitivity to NADH inhibition.
As predicted this mutant protein was unstable as it could no longer interact with Pin1.
As predicted, in mutant embryos we saw a large increase in cells with GFP-positive nuclei in tracheal branches (Fig. 1L,N), with this sometimes being observed in pairs of cells yet to intercalate (Fig. 1N, arrow).
