Sentence examples for mutants as indicated from inspiring English sources

Protein expression and purification protocols for Kv7.2 helices AB and the helices ABCD fused to GST (GST-AB and GST-ABCD), the deletions and mutants (as indicated in the figures), and for CaM have been described in detail elsewhere36,37.

We first looked at the transcription of the different constructs that, as expected, was similar in all mutants as indicated by semiquantitative RT-PCR (Figure 1C).

The hairpin transgene remains silenced in both dcl mutants, as indicated by the presence of GUS siRNAs and little accumulation of full-length ΔGUS-SUG mRNA (Figure 2B, lanes 11 13).

Using Lipofecatamine 2000 on DIV 4, neurons were transfected with GFP alone or co-transfected with GFP and LRRK2 (WT and mutants as indicated) in a 1∶9 ratio.

HEK293T cells were transfected with an expression vector for A3F-C-V5 plus a control vector (Fig. 6D, lane 1), the expression vector for WT Vif-myc (lane 2), or one of various Vif mutants, as indicated (lane 3, 4, 5, and 6).

Huh-7 cells were transfected with plasmids encoding tsNTCP, hNTCP, mkNTCP, or NTCP mutants as indicated.

Opsin T242B could bind all the arrestin Trp mutants tested to some degree, as indicated by their inhibition of Gt activation.

HEK293T cells were transiently transfected with empty psg5 vector and the mutants m1, m3, m7, and m9 as indicated.

On the other, the long MD simulations of the sTKD dimer in wtEGFR and T766M-EGFR show more stability for the single mutant (as indicated by root-mean-square fluctuations, Supplementary Fig. 19a) and suggest that T766M stabilises the sTKD dimer through a network of interactions (Fig. 6f (right), Supplementary Fig. 19b).

The levels of Syb and Brp were significantly reduced in the wnt2 mutant as indicated by immunocytochemistry (Fig. 2).

HEK293T cells were transfected with SIVagm△Vif and with an A3C expression vector plus a control vector, an expression vector for wild-type (WT) Vif, or a Vif mutant, as indicated in Fig. 1B.