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As shown in Table 1 and Figure 3C, while the VH genes amplified from CD19+CD27−IgD+ cells in the control group had a low number of mutations, as expected (average 3 mutations per gene; mutated in 1.2% bp sequenced), the VH genes from patient CD19+CD27−IgD+ cells had a significantly higher number of mutations (20 mutations per gene, P<0.001; mutated in 6.3% bp sequenced).
No transamidating activity was detected with the W241A and C277S mutations as expected (Fig. 3a).
Whole genome sequencing revealed a few genomic mutations, as expected.
These 17 mutations were G/C-to-A/T mutations, as expected for EMS [ 17].
Larger mutations, as expected under genomic instabilities, are also expected to be less likely to generate adaptive change (Orr 2005).
The mutDNA samples have a high frequency of single- and multi-nucleotide codon mutations, as expected from the codon mutagenesis procedure.
Similar(47)
The observed high frequencies of FGFR3, TP53, and PIK3CA mutations were as expected.
Given the sequences of the seven targets, the distribution of mutations was as expected.
Results of the TP53 mutation analysis were as expected from data in the scientific literature; however, the absence of KLF6 miscoding mutations in our patients and in the three human prostate cancer cell lines was unexpected.
(9)– (14, 14) The majority of variants identified were transitions leading to nonsynonymous mutations (Fig. 2b,c), as expected.
This indicates that the control ODN does not induce repair of the point mutation, as expected.
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