Sentence examples for mutants as determined from inspiring English sources

The Metabolic flux rates of 31 central carbon reactions in 24 E. coli metabolic deletion mutants as determined by C-tracer experiments were reported by Ishii and [ 25].

Another three samples had small differences in melting behavior compared to that of non-mutant, but they were not mutants as determined by sequence analyses.

Strikingly, knockdown of ACS2 almost completely restored age-associated autophagy of ach1 mutants, as determined by the frequency of cells with GFP-Atg8p-positive vacuoles and the levels of liberated GFP reflecting autophagic flux.

We performed alanine mutagenesis of these 16 residues individually, expressed them recombinantly in Escherichia coli, purified 450 kDa complexes for all mutants as determined by gel filtration analysis, and tested binding to NCOA4 in NCOA4-GST pulldowns.

Nonetheless, much lower activity than PKM2 WT was detected with this mutant as determined by pyruvate kinase assay.

Under these conditions, biomass production of the ∆apcE mutant, as determined from OD750, was approximately 1.6-fold higher than that of GT after 15 days of cultivation with 50 μmol photons m−2 s−1 illumination (0.84 ± 0.07 and 1.32 ± 0.01 g-cell-dry weight L−1 for GT and the ∆apcE mutant, respectively) (Figure 2).

(B – E ) AtLYK5K395E but not AtLYK5ΔKD (1 320 aa) complemented the Atlyk5-2 mutast as determined by chitin-triggered ROS production.

Lada and the ST mutant as determined by Northern hybridization of total leaf RNA (data not shown).

A recent study also notes an increased number of C-terminal to N-terminal contacts in the absence of lipids for the E46K mutant, as determined by solution NMR paramagnetic relaxation enhancement measurements (34).

This pattern is also in agreement with the observed decreased flux of carbon through the lower part of plastidic glycolysis from phosphoenolpyruvate and downwards in developing embryos of the wri1 Arabidopsis mutant as determined by C metabolic flux analyses [ 36].

Antagonists (5 μL) were applied, and after incubation for an additional 20 min at 37 °C, 20 μL of receptor specific agonists was applied and the calcium flux monitored using a Flex Station (Molecular Devices) over a period of 1 min. In all cases, the EC80 specific for the individual mutant as determined in previous experiments was reported.