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Exact(13)
The replacement of this eight-residue segment with the corresponding llama sequence gave a chimeric mutant which shows significantly decreased fluctuations.
To our knowledge, the Otx2 CKO mouse is the only mutant which shows defects of both rods and cones in the retina from early developmental stages.
In this context, our analyses included the snf7 mutant, which shows defective MVB formation [31], and the sec4-2 mutant, due to the implication of the SEC4 gene in the secretion of Golgi-derived vesicles in yeast cells [17], [32].
The present study is a more comprehensive evaluation of the effect of ambient light on photoreceptor degeneration in this type of mutant, which shows that the protective effect of ambient light can be very strong in 52H mice where the levels of Y99C GCare are not as high as they were in the L53 mice[17].
The Sced mutant, which shows a strong phenotype in the early syncytial divisions in Drosophila [18], allowed us to test the effect of a reduced actin cap area, from where dynein could pull on MTs, without the use of any drug.
This model is supported by our analysis of the disulfide cross-linked A89C/I94C mutant, which shows cleavage kinetics similar to those of the A92P mutant.
Similar(46)
The first DGDG synthase in Arabidopsis, DGD1, was revealed by isolation of the dgd1 mutant, which showed 90% reduction in DGDG content (Dörmann et al., 1995).
Additionally, smf-1 eh5) smf-1 eh5ly mutant whish showed a significanthen-dependent depletionlyf Fe content, despite the overexpression of smutantn this context.
Except the K14A mutant which showed a loss of approximately 18% of the secondary structure, all mutants showed similar values of the secondary structural components as the wild type enzyme (Figure 3).
Further evidence that AFB5 contributes to the dicamba response comes from the double, tir1-1/afb5 mutant, which showed stronger resistance to dicamba than either the tir1-1 or afb5 single mutants.
In addition, we found that all mutant constructs maintained their ability to bind DNA, except the N178Q DNA binding domain mutant which showed significantly reduced DNA binding as demonstrated by electrophoretic mobility shift assays (EMSA; Figure S1B; ref. 18).
Related(20)
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com