The map shows that the constitutively active mutant, which lacks the C-terminal domain involved in osmosensing, is trimeric like wild-type BetP.
Intracellular concentration of this metabolic intermediate could be increased more than 20 times compared to the wild type by overexpressing GPD1 encoding the glycerol 3-phosphate dehydrogenase in a gpp1Δgpp2Δ mutant which lacks both isoenzymes of glycerol 3-phosphatase.
The Adenovirus (Ad) dl309 mutant, which lacks several E3 region genes, has been used as the backbone for a number of replication selective cytopathic Ads designed to treat tumours.
Phytochrome signaling affects this day-length-dependent conversion of Hd1 activity because this conversion is not observed in the phytochrome-deficient mutant; flowering of the double mutant se5 hd1 (which lacks both phytochrome and functional Hd1) was slightly later than that of the se5 single mutant (which lacks only phytochrome) [28].
The tail of the α-heavy chain, identified by analyzing the oda11 mutant, which lacks α-heavy chain, extends from the AAA ring tilted toward the tip of the axoneme and towards the inside of the axoneme at 50°, suggesting a three-dimensional power stroke.
There is no detectable tyrosine phosphorylation of the M7 mutant which lacks an intracellular domain (Figure S1) or the YΔF mutant which lacks critical tyrosines within the ICD (Figure 3A).
As expected, no labeling was observed for the double C22/28S mutant, which lacks cysteine residues (Fig. 3B, lane 4).
This weak superhydrophobicity is not present for the mixta mutant, which lacks the conical-celled surface corrugation.
DNA from vegetative tissue of a N. crassa wild type (dim-2+) and of a dim-2 mutant (which lacks all detectable DNA methylation; [12], served as controls.
As shown in Figure 2A, VHL stability was significantly increased in the presence of wild type PIASy but not its mutant which lacks VHL-binding ability.
The Δ155 165 mutant, which lacks residues 155 165 corresponding to the ER peptide, increased microtubule assembly, and decreased both microtubule disassembly and tubulin aggregation.
