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However, the secondary inoculation for the transfer of a virus from C. quinoa to a systemic host plant is somehow difficult especially for a viral mutant, which does not form distinct local lesions.
As predicted, the P4 mutant, which does not bind to YopJ, cannot be acetylated (Figure 4).
The BCR63-ABLF1081E mutant, which does not associate with F-actin (Figure 6F), remains cytoplasmic in the absence of drug treatment (Figure 6D).
We further prepared the NIX mutant, which does not contain the BH3 domain (designated NIXΔBH3), in order to examine the role of the BH3 domain in the interaction of NIX with Mieap.
Although the ΔrcpA mutant did not respond to IL-1β, it bound biotinylated IL-1β as much or slightly more than the Δflp1-flp2 mutant, which does not produce fimbriae but has intact outer membrane Tad machinery.
Likewise, apart from a recent article focusing on the pro-inflammatory effects of an α-Syn double mutant which does not exist in nature [54], a comparative study of wild-type α-Syn and pathologically relevant α-Syn mutants is still lacking.
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In contrast, significant amounts of the S286E mutant, which did not undergo cofactor-activated phosphorylation, were recovered in the postnuclear supernatant even in the presence of Hes1 (Fig. 6Ba, cf. lanes 7 and 8).
This was in contrast to what was observed for the constitutively active and ligand-independent TPR-TRKB mutant, which did transform RIE-1 and RK3E cells and suppressed anoikis in the absence of exogenous BDNF, consistent with our previous report [25].
On the other hand, the csp-2 ok1742) csp-2 ok1742h did not display ectopic germutant deaths, had a normal brood size.
Furthermore, except for the mild exo70A1-1 mutant (which did not differ from wild-type), the time cortical cells of the exocyst mutants spent in the EZ was actually longer than that observed for wild-type Col-0.
Notably, the ATPase-deficient Kar2-T249G mutant (which did not enhance growth of cells during oxidative stress; Figure 7) did not show an enhanced ability to prevent aggregation of rhodanese, supporting a correlation between a greater holdase capacity of BiP and enhanced viability during oxidative stress.
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