Overexpression of Gαq protein increases Ang II-induced signaling for all of the tested receptors except the DRY/AAY mutant, which remains practically uncoupled.
For example, consistent with our observations, the STAT5b VVVI mutant, which remains primarily cytoplasmic and does not accumulate significantly in the nucleus even after prolactin stimulation, is capable of inhibiting STAT1-mediated activation of the interferon regulatory factor-1 promoter at an extranuclear level [35].
In contrast, with an iglC mutant of LVS, which remains trapped into the phagosomal compartment, or with inert particles, nucleolin/bacteria co-localization remained almost constant.
We propose that CBS1 is required for normal peroxisomal biogenesis, but that this mutant is readily suppressed, the mechanism of which remains unclear.
In addition, we found that expression of dFMRP mutants lacking RNA-binding motifs (ΔKH or ΔRGG) still induces dFMRP granules (Fig. 1C, Figs 3, 4), further indicating that dFMRP granule formation indeed requires the N-terminal PP domain of dFMRP which remains intact in these mutants.
Another major phenotypic class of developmental mutants – those that form aerial hyphae but do not sporulate efficiently – identified the whi genes, so-called because the mutants fail to accumulate spore pigment in their aerial mycelium, which remains white on prolonged incubation (Hopwood et al., 1970; Chater, 1972).
Unlike wild-type or (66delD Cx31, the expression of the EKV mutants are associated with elevated levels of in vitro cell death (21– 23) through a mechanism which remains to be elucidated.
Since RT dimer is the stable form which remains resistant to the proteolysis, we tested whether treatment of dimerization enhancer could reduce the mutant RT proteolysis.
Instead, that which remains behind grows larger.
Which remains the most improbable?
The BCR63-ABLF1081E mutant, which does not associate with F-actin (Figure 6F), remains cytoplasmic in the absence of drug treatment (Figure 6D).
