plants of the accession Columbia (Col-0), except for the mp-B4149 mutant, which has the Utrecht background41,66.
Mixing the Val70Thr/Val71Thr double mutant, which has inherently low potential, with the fibril forming α-synucleins, WT and Ala53Thr, greatly reduced their fibril formation and aggregation.
The example considered is the trafficking of carbon in an Escherichia coli mutant, which has pyruvate kinase activity deleted for the purpose of eliminating acetate production.
Our rational amino acid substitution approach resulted in the construction of the Ser114Ala/Phe355Leu mutant, which has an 11-fold decrease in oxidase activity and 2.8-fold increase in dehydrogenase activity compared with wild-type GOx.
Hd3a expression under SD conditions is significantly up-regulated in an early-flowering photoperiodic sensitivity 5 (se5) mutant, which has a mutation in the gene encoding heme oxygenase, which is required for phytochrome chromophore biogenesis [27, 28].
The novel imazethapyr tolerant mutant which has been developed indigenously has opened up the possibilities of its extensive usage, without fear of infringement of any IPR, in the public rice breeding programmes in India and elsewhere.
Unlike the Grm1 mutant, which has excessive climbing fibers [36], the Inpp4awbl mutant climbing fibers develop normally until P15 when elimination begins.
A similar predominance of proteasomal degradation occurred in case of the Y950F mutant, which has an impaired IRS-1 binding [38], [39].
The D32E mutant, which has the same functional group as the native residue at this position has a very minimal effect on the Kcat of the mutant enzyme.
Deletions in the promoter of this gene cause abnormal lens development in the aphakia mouse mutant, which has only rudimentary lenses [24], [25].
By comparison, we looked at the fate of intracellular nucleolin in human macrophage-like cells infected with a Francisella iglC mutant, which has a severe defect in phagosomal escape and intracellular replication [19], [20], [22].
