In contrast, the S298E mutant, which undergoes an apparently normal cofactor-activated phosphorylation, has an intact anti-neurogenic ability.
We therefore introduced the SPA constructs also into the spa1 spa2 spa3 triple mutant which undergoes constitutive seedling photomorphogenesis in darkness, while it develops normally as an adult plant [ 13, 15].
This hypothesis is consistent with our findings that the fast cycling mutant Cdc42 F28L, which undergoes spontaneous activation without the assistance of GEF proteins, does not reconstitute Ca2+ mobilization in B6A4C1 cells (M.M.W., unpublished observations).
As expected, in the GLP-1 loss-of-function mutant glp-1 lf), glp-1 lfdergoes early differentiation of mitotic cells and early entry into meiosis, whichserved a shift of the H3K23me3 signal towards the distal tip of the gonad.
The per ; tim double mutant displays the per phenotype, and the cyc ; per double mutant displays the cyc phenotype, as would be predicted from the circadian clock transcriptional feedback loop, which undergoes circadian rhythms in this tissue.
This is very similar to the behavior of osk mRNA, which undergoes fast, direct movements 4 5 times more frequently in wild-type oocytes than in EJC, TmII, and stau mutants.
This is entirely consistent with a previous report showing that the APP M596I mutant, which cannot undergo β-secretase cleavage, increases the resistance of APP FAD mutant-expressing cells to apoptosis.
Cells with mutant p53 genes are susceptible to cancer-causing mutations and survive UV radiation more often than do normal cells, which undergo programmed cell death when damaged by sunlight.
In contrast, significant amounts of the S286E mutant, which did not undergo cofactor-activated phosphorylation, were recovered in the postnuclear supernatant even in the presence of Hes1 (Fig. 6Ba, cf. lanes 7 and 8).
Activated Lyn and Fyn (SFKAct) constructs were generated by activation loop autophosphorylation of Y527F mutants, which cannot undergo C-terminal tail autoinhibitory phosphorylation.
Similarly, the C. albicans cph1Δ/efg1Δ mutant, which is unable to undergo the transition into filamentous hyphae, recovered filamentous growth post 19 cycles of cocultivation with macrophages (Wartenberg et al. 2014).
