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The ELG1 gene was identified in yeast as a mutant that causes enhanced levels of genomic instability [3], [4], [5].
We next adapted this technology to conduct a small molecule screen and identified compounds that altered the intracellular accumulation of the human aggregation prone mutant that causes liver disease in α1-antitrypsin deficiency.
Subsequently, the phenotype was observed in a Sec61p protein translocation mutant that causes defective ovulation [ 9].
Therefore, one could potentially choose the mutant that causes the greatest level of chromosome nondisjunction during meiosis.
This finding shows that PP2Cs are an important mechanism that controls Ca2+ sensitivity priming in guard cells and identifies a first mutant that causes constitutively primed Ca2+ sensitivity in guard cells.
Combined with biochemical and genetic evidence, our electrophysiological data provide strong evidence for newly recognized molecular mechanisms and a first genetic mutant that causes constitutive Ca2+ sensitivity priming in a plant cell.
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Importantly, 2D-gels results on replication bubbles were further and fully confirmed in analysis of origins by BrdU incorporation and hybridization of BrdU-labeled DNA on Affymetrix tiling arrays on mrc1 and rad53-11 mutasts, as well as on the mec1-1 muthat that cause fork stalling and early activation of late origins, respectively [40], see Figure S5.
Although rpm-1 mutants are reported to move normally, rpm-1 (lf) has a synthetic synaptic phenotype with syd-2 (lf) mutants that causes impaired locomotion (Liao et al. 2004; Noma et al. 2014).
The authors used a V. carteri mutant, which carries a mutation that causes somatic cells to redifferentiate as asexual reproductive cells.
Hypotonicity triggered on average a 130% increase in the currents from mutants that cause the mildest form, BO (Fig. 3d).
The GSK responses of the mutants that caused the mildest forms of MD were statistically indistinguishable from those that caused SMDK (Fig. 4d).
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Justyna Jupowicz-Kozak
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