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In this study we applied rational design to ManBK by using its crystal structure as a basis and identified the Y216W mutant that shows great potentials in industrial applications.
Here we report on the characterization of an exo knock-out mutant that shows dwarfism.
We describe karneol (kar), a new iridophore-deficient mutant that shows an adult-specific phenotype similar to weak rse alleles.
We previously identified extra-small sister1 (xs1) mutant that shows decreased final cell area in leaves [ 17].
In fact, it has been shown that an erg25 mutant that shows normal growth in iron-replete medium is hypersensitive to iron-limitation (Li and Kaplan 1996).
Overexpression of extended-C/EBPα, in particular of the S299D mutant that shows augmented nucleolar retention and Pol I activation, results in a pronounced increase in cell volume.
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P334S was the only mutant that showed increased heterologous expression levels and thermal stability in both 2B6 and 2B11.
Nevertheless, in the XacFhaB mutant that showed faster movements in swarm plates, flagellar protein expression was lower as compared to the wild type and XacFhaC mutant strains (Figure 6A).
The higher activity is due to the fact that we used 2 ng/cm2 of each toxin, one being wild type and the other being either the D136N or A140K mutant that showed reduced toxicity but remain active (Table 1).
The tgs1 deletion mutant, that showed an inability to accumulate TG, was not able to develop antibiotic tolerance at the level of the wild type strain and complementation restored antibiotic tolerance.
The second mutant that showed a germination defect was erg6Δ.
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