The isolation of E. coli mutants that tolerate high levels of Gp39.2 will perhaps help locate the putative GroEL Gp39.2 interface.
To distinguish beneficial SNPs in XI, we sequenced the metagenome of Xp to a depth equivalent to 140 sequence coverage of X514 genome, the pool of mutants that tolerated 2% ethanol, reasoning that non-synonymous mutations with higher mutation frequency (percentage of mutated reads to all reads at a single-base locus in the X514 mutant community) were more beneficial (Additional file 10B).
"This is a city that tolerates dissent.
Moreover, the survival of mutants that are sensitive to aerobic conditions (Hpx− dps and recA fur), presumably because of their inability to tolerate ROS, seems to depend on CO2 concentration.
Synthetic dosage lethal (SDL) screens in which mutants that cannot tolerate overexpression of kinetochore proteins have identified chromosome stability genes, many of which are not components of the kinetochore (e.g., chromatin-modifying or tubulin-folding proteins) [17] [23].
Someone able to tolerate that can tolerate a lot," Hakan said.
The Ime2 DFGx mutant shifts from a strong +1R preference to an expanded specificity that tolerates both proline and arginine at the +1 position.
It is not a game that tolerates flopping.
With 240 uM iron chelator, the fur mutant displayed a much shorter lag phase than MR-1 and reached a higher cell density at stationary phase, suggesting that the fur mutant better tolerated the iron depletion stress.
They reported that both the wild type and the esk1-1 mutant can tolerate freezing only after a cold acclimation period and that without acclimation, the two genotypes avoid freezing by delaying or preventing frost damage.
