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The novel mutant lines are useful genetic resources for production or future cowpea breeding programs in Namibia or similar environments.

These two mutant lines are the same as those studied by Yamaoka and Leaver.

Earlier studies [14] reported that the 05342 and 08941 mutant lines are flight impaired, and this effect worsened with age.

Because 21 nt ta-siRNAs are produced by DCL4 [21] [23], this result confirms that these dcl4 mutant lines are highly deficient for DCL4 activity.

Transgenic and mutant lines are listed in supplementary material Table S5.

Details of all mutant lines are shown in Additional file 8: Table S4.

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The homozygous overexpressor and knockout mutant lines were confirmed using western blot analysis.

Amylose content, seed morphology, and starch granules of pul mutant lines were essentially the same as those of wild-type plants.

The panicles from WT and mutant lines were harvested post-maturity (40 DAH) and air-dried for 2 weeks before analyses.

The enzyme activity in the mature leaves of the mutant lines was lower by 28.8% relative to the WT, suggesting a reduction in soluble acid invertase activity in the mutants, with observed recovery in the C4 line (Fig. 1e).

Mutant lines were maintained in the AB* and WIK backgrounds.

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