It was shown microscopically that mutant cells are smaller, than wild type cells.
This mutation has emerged following treatment with imatinib, and therefore mutant cells are resistant to imatinib [15].
However, due to a slower transition from lag phase to exponential phase, mutant cells are outcompeted by the WT in every co-culture treatment.
Indeed on root cross-sections 0.5 cm from the apex, and on polar views of these sections, epidermal, exodermal and sclerenchyma of mutant cells are not gathered in 3 well-defined layers.
Rad21 heterozygous mutant cells are defective in homologous recombination (HR -mediated gene targeting and sister cHR -mediatedhangene
Consistent with this, analysis of pico mutant clones indicates that the mutant cells are extruded from the epithelium [4].
Cds1 null mutant cells are extremely sensitive to the deleterious effects caused by HU and methyl methane sulfonate (MMS) [31].
We next considered whether ph0 mutant cells are dying in which case extrusion might reflect a response to cell death.
Also, using both approaches, only ph mutant cells are expulsed, indicating the cell autonomous nature of the phenotype.
However, these triple mutant cells are inviable at temperatures that otherwise support ctf7 rad61 mutant cell growth (Figure 3).
As expected, wildtype and rad61 cells exhibit robust growth at all temperatures while ctf7-203 mutant cells are inviable at 30°C and above (Figure 2).
