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Given Bj mutant phenotypes are reminiscent of ciliopathies, these findings suggest Pk1 may also regulate ciliogenesis.
An essential control for genetic manipulation of microbes is the regeneration of the wild-type state and phenotype to validate that any mutant phenotypes are 'on target'.
Methods to study mutant genotypes that lead to mutant phenotypes are discussed, particularly the use of model organisms and mutant proteins to study protein interactions that may be important for development of disease.
This suggests that sqh mutant phenotypes are chiefly caused by sequestration of myosin into inactive aggregates.
The activation tagging strategy has been successfully used [7] as mutant phenotypes are caused by overexpression rather than knock-out of the targeted gene.
Despite the fact that the D-cbl mutant phenotypes are similar to the ones described for argos, gap1 and sprouty, we noticed at least two phenotypes which appear to be specific for D-cbl.
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Modeling of wild-type and mutant phenotypes is predictive; although closure in myospheroid mutants ultimately fails when the cell sheets rip themselves apart, our analysis indicates that beta(PS) integrin has an earlier, important role in zipping.
In both lines, the mutant phenotypes were fully rescued.
However, no mutant phenotypes were found in the pancreatic tissues of pc mutants.
Other genes with mutant phenotypes were added to the map over the years.
The mutant phenotypes were maintained after one generation of clonal propagation and the plants died before further propagation.
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