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In addition, all septin mutant cells produced bipolar growing filaments.

After seven days in the appropriate medium, ERRα mutant cells produced a greater number of differentiation foci as estimated by alkaline phosphatase (ALP) staining (Fig. 3A).

Instead, condensin mutant cells produced a severe defect in chromosome segregation shortly after the initiation of mitosis (Fig. 6B,C, right).

Our results showed that both wild type and mutant cells produced significantly larger capsule (P < 0.0001) when they were cultured on medium with inositol as a carbon source than on medium with glucose as a carbon source.

However, in contrast to what was observed in the VHG-optimized growth medium, in which a slight reduction in final ethanol production was observed in Δ ppa1 cells, these mutant cells produced the same amount of ethanol from the WSH as wild-type cells (Table 4 and Figure 3).

Similar(55)

(E ) gefE − mutant cells produce more spores than Ax3 wild type cells after G− growth.

This may reflect a possible feedback mechanism in which the mutant cells produce MDM2/P-MDM2 in an attempt to rescue p53 from further degradation.

In contrast, the introduction of ezrin-T567A and ezrin-R579A mutants into cells produced dramatic changes in cell morphology, characterised by significantly higher cell perimeters (Fig. 5A), consistent with the formation of membrane projections (Fig. 5B).

The targeting construct was introduced to mouse embryonic stem (ES) cells and mutant cells were produced by homologous recombination.

Given the high rate at which mutant cells are produced in at least some tumours (Loeb 2011) and associated genetic instability (considered an enabling characteristic for several of the cancer hallmarks, Hanahan and Weinberg 2011), this allows ample opportunity for the emergence of heterogeneous cell populations containing chemoresistant clones in neoplasms prior to their detection.

As shown in Figure 8 figure supplement 1, Gli1-GFP expression was drastically reduced in Nfe2 mutant mice supporting the idea that platelet mutants have reduced dentate cells produced from Shh activation.

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