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We also observed that clones of TOR mutant cells generated in otherwise wild-type eyes were massively underrepresented.
Finally, we also examined the overall morphology of clonally related groups of wild-type and FasIII mutant cells generated in wing imaginal discs (Fig. 5C-E).
This likely explains why we did not observe ectopic S-phase cells in cic mutant cells generated in rbf1 120a heterozygous eye discs despite the elevated level of Cyclin E expression (supplementary material Fig. S1).
Furthermore, our analyses do not support the recent proposal that Utx would regulate cell proliferation in Drosophila as Utx mutant cells generated in wild-type animals proliferate like wild-type cells.
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Thus, our results suggest a high rate of fork abnormalities in swi1Δ and swi3Δ mutant cells, generating ssDNA regions near the replication fork, which induces accumulation of HJ-like structures [19], [20].
In standard colony-forming assays in vitro, mutant spleen cells generated 32% more erythroid colony-forming units (CFU-Es) than wild-type cells and, although they were slower than controls, they eventually generated 50% more erythroid burst-forming units (BFU-Es) than controls (Fig. 1D).
(A ) Control DT clone of GFP-expressing cells with no effect on morphology or expression of Delta; (B-–F' ) Clones of sal mutant DT cells generated bulges, apparently sorting out, and lacked Delta expression (B, B' ), ectopically expressed vein-lacZ (C ) and Knirps (D ), not normally expressed by DT cells, but not Cut (E, E' ) and activated the NRE- lacZ reporter (F, F' ).
Marked clones of mutant cells were generated by Flp-mediated mitotic recombination subjecting flies once to a 35°C heat-shock for 30 minutes (Act5C<CD2>Gal4) or three times to a 38°C heat shock for 30 minutes (FRT42D Sans245).
Extensive studies of cdc13-1 mutant cells have generated much insight into cellular responses to telomere uncapping and DSBs.
Clones of mutant cells were generated by FLP-mediated mitotic recombination as described (Mukai et al., 2011).
Clones of mutant cells were generated by the FLP-FRT method and induced in first/second-instar larvae by heat shock at 37 °C for 2 h.
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