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While wild-type cells were able to form dark, stellate melanophores (n = 13; Figure 7D), enz mutant cells formed punctate melanophores (n = 5; Figure 7E).
Wild-type cells and SJ mrc1 or SJ cds1 mutant cells formed colonies with hypha (Fig. 1).
In reconstruction experiments, doubly mutant cells formed colonies by day 2 when plated with or without a tester lawn.
Similarly to what we found for the deletion of SSA1 and SSA2, the hsp104Δ mutant cells formed the age-associated protein deposit more rapidly during aging.
Thus in the presence of the tester, the added singly mutant cells formed colonies whose appearance is delayed 1 4 days by the presence of a tester lawn.
On closer inspection, it was evident that mutant cells formed rounded clusters with a paucity of lamellipodia or filopodia, likely affecting their capacity to migrate (Fig. 1A, Supplementary Material, Movies 1 3).
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Under these conditions, wild-type cells form aggregative, rdar morphotype colonies, whereas csgD deletion mutant cells form smooth colonies that lack EPS production (Figure 1; [9], [9]).
Even though mutant cells form aerial mycelia and spores, we consistently found that the yield of spores was only about 1/5 that of wild type cultures after one or two weeks of growth.
In this approach, mutations are induced selectively in specific tissues, where genetically affected mutant cells form tumors in an otherwise wild type larval body.
The scrib- mosaic clones, however, deviate significantly from previous studies in homozygous animals: instead of displaying grossly neoplastic overproliferation, the mutant cells form small disordered foci that never extend beyond original clonal region of expression.
That is, each double mutant cell formed a visible colony (∼10 cells) within 2 days on selective medium.
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