T cells recognize pMHC complexes through the TCR-CD3 cluster.
To address whether LCL-stimulated T cells recognize lytic cycle antigens of EBV, mini-LCL incapable of expressing lytic cycle proteins were established by infecting B cells with a genetically engineered mutant strain of EBV and used as T cell targets [22], [34].
By dot blot, the anti-Rtls signal was reduced in mutant cells even though the antibody should recognize both wild type and truncated Rtls isoforms.
Competition to eliminate pre-neoplastic tumors makes use of signals and receptors also used in patterning the nervous system including Slit/Robo2 and Sas/PTP10D to recognize and extrude clones of mutant cells, at least where local epithelial cyto-architecture is favorable.
As the basally localized IdU+BrdU− nuclei in the mutant were not recognized in G2/M by PH3, it suggested that these mutant cells might arrest in the basal S phase zone due to the stalled DNA replication.
In wild type neurons in mosaic eye discs DG-C antibody staining was localized to the apical surface but was absent from the Dg mutant cells (Fig. 1A, C; F H), confirming that the antibody selectively recognizes DG.
Indeed, 25.8% of myt1 1 mosaic mutant germaria contained no myt1 1 mutant GSCs, but did carry myt1 1 mutant germ cell cysts (recognized by the absence of β-gal signals) at 4 days ACI (Fig. 5D); therefore, at least one myt1 1 mutant GSC had been lost from these germaria.
You can say there is a statistically significant effect for your chemical reducing the firing rate in the mutant cells.
But you can't say mutant and normal cells respond to the chemical differently: to say that, you would have to do a third statistical test, specifically comparing the "difference in differences", the difference between the chemical-induced change in firing rate for the normal cells against the chemical-induced change in the mutant cells.
In that respect, SoBig's variants have acted more like mutant cells in a cancer than a virus, computer security experts say.
