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The major difference between the two studies is that yeast mutant cells express tub2-S172E as their unique source of β tubulin, while in mammalian cells, the mutant S172E β tubulin was expressed among normal β tubulin isoforms.
This indicates that in the presence of smoke derivatives LHON mutant cells express the genes regulating xenobiotic detoxification at a higher level compared with controls.
Furthermore, in contrast to the wild-type cells, the UAS- dpn RNAi; E spl) XP mutant cells express dMyc (Fig. 5A A″).
DOI: http://dx.doi.org/10.7554/eLife.02443.004 Arrested His C mutant cells express high levels of mitotic Cyclin B suggesting a cell cycle arrest in G2 phase of cycle 15 (G215) before mitosis (Günesdogan et al., 2010; Lehner and O'Farrell, 1990; Figure 2A).
In stage 7 8 awd clones over-expressing NICD (from the UAS-NICD transgene [ 53]) (GFP-positive cells in Figure 4A), 60.5% of mutant cells express Hnt (199 out of 329 cells) (GFP-positive cells in Figure 4B), representing a significant rescue of the lack of Hnt expression phenotype.
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Since filamentation of efg1Δ mutant cells expressing EED1 was observed, we analyzed the expression of the hyphal-associated genes ECE1 and HWP1 and the hyphal regulatory gene UME6 in wild type, efg1Δ and efg1Δ + pTET-EED1 cells.
First, because WG expression can be induced within mutant cells expressing only Ofut1 R 245 A, it indicates that, as during embryonic neurogenesis, modification of Notch with O-fucose is not required for it to function as a receptor.
(C) Wild-type or S174 mutant cells expressing Atg9-GFP and Cherry red-Atg8 were transferred to SD-N medium for 2 h and observed by confocal microscopy.
(A) Wild-type or S174A mutant cells expressing Atg9-GFP were transferred to nitrogen starvation for 0 h or 2 h and observed by confocal microscopy.
To determine if Fe-S cluster assembly in Pri2 is sensitive to oxidative stress, we carried out 55Fe labeling and Pri2 immunoprecipitation assays in wild-type and sod1Δ mutant cells expressing transformed Myc-tagged Pri2.
For the localization of endogenous Sufu proteins in Gli mutant cells expressing various GFP-tagged Gli protein variants, ciliated cells with GFP fluorescent signals were scored.
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