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Since filamentation of efg1Δ mutant cells expressing EED1 was observed, we analyzed the expression of the hyphal-associated genes ECE1 and HWP1 and the hyphal regulatory gene UME6 in wild type, efg1Δ and efg1Δ + pTET-EED1 cells.
First, because WG expression can be induced within mutant cells expressing only Ofut1 R 245 A, it indicates that, as during embryonic neurogenesis, modification of Notch with O-fucose is not required for it to function as a receptor.
(C) Wild-type or S174 mutant cells expressing Atg9-GFP and Cherry red-Atg8 were transferred to SD-N medium for 2 h and observed by confocal microscopy.
(A) Wild-type or S174A mutant cells expressing Atg9-GFP were transferred to nitrogen starvation for 0 h or 2 h and observed by confocal microscopy.
To determine if Fe-S cluster assembly in Pri2 is sensitive to oxidative stress, we carried out 55Fe labeling and Pri2 immunoprecipitation assays in wild-type and sod1Δ mutant cells expressing transformed Myc-tagged Pri2.
For the localization of endogenous Sufu proteins in Gli mutant cells expressing various GFP-tagged Gli protein variants, ciliated cells with GFP fluorescent signals were scored.
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As shown in Figure 2B, swi3-D84H (from swi3-E31), swi3-W95R (from swi3-E40), and swi3-L112R (from swi3-NBT7) mutant cells expressed somewhat lower amounts of Swi3-TAP protein.
The major difference between the two studies is that yeast mutant cells express tub2-S172E as their unique source of β tubulin, while in mammalian cells, the mutant S172E β tubulin was expressed among normal β tubulin isoforms.
While samples derived from clpP mutant cells expressed wild-type levels of β-galactosidase (data not shown), β-galactosidase activity was reduced approximately two fold compared to wild-type in clpX mutant cells.
All the mutant cells expressed NRE- lacZ.
The sal mutant cells expressed Kni and vein but did not express either Delta or Cut.
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