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Results of the growth experiments show that mutant cells have double the exponential growth rate of the WT in monoculture.
Approaches for measuring Pig-a mutant cells have focused on peripheral red blood cells (RBCs) and reticulocytes (RETs) from rodents.
Western blotting analysis demonstrated that the protein lysates extracted from the mutant cells have significantly increased amount of LC3B-II when compared to the wild-type cell lines.
Specifically, mutant cells have less than 10% of normal patterns, with the aberrant segregated and fragmented patterns representing the majority of the mitotic cell population.
BRCA1 mutant cells have a 100 fold increased sensitivity to mitomycin C [41], emphasizing the importance of BRCA1 in the cellular response to the DNA crosslinking agent.
This conclusion is entirely consistent with studies in wing epithelium where groups of Vang and fz mutant cells have a domineering non-autonomous effect and cause neighboring wild-type cells to mispolarize relative to the main proximal-distal axis.
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Normally compact and crescent-shaped, the nucleoli of the mutant cells had become enlarged and fragmented.
Moreover, the age3 mutant cells had a clear delay of endocytosis compared to wild-type cells.
The overexpression of Upd in ept mutant cells has also been shown to occur by a Notch-dependent mechanism [6].
For missegregation to occur, mis15 and mis17 mutant cells had to be continuously maintained at the non-permissive temperature from the previous mitosis.
Compared to wild-type cells it took about 20 minutes longer (this corresponds to about 1/3 of the doubling time) until 50% of the mutant cells had taken up FM4-64 inthethe vacuolar membrane (Fig. 3C).
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