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Mutant animals express a fully functional receptor with a fused C-terminal eGFP (DOR-eGFP) in place of the native receptor and show no detectable alteration of behavior and responses to drugs.
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To test this, we counted the number of fluorescent foci in hif 1 ia04) mutant animals expressing YFP polyQ35 after exposure to 1000 ppm O2.
To determine the impact of Wrn and Safb1 proteins on the health of mice, mutant animals expressing Wrn protein lacking part of the helicase domain (referred as Wrn Δ hel/ Δ hel hereafter) were crossed to Safb1-null mice.
We found that both vhl-1 ok161 vhl-1 ok161sand4) mutant animals expressing YFP::polyQ35 accumulate the samutantber of foci animals-typexpressing when exposed to 1000 ppm O2 (Fig. 4C).
The size and number of the ommatidia in the eyes from control wild-type (ey-dm +) was compared to that from ey-dm P0 or ey-dm 4 mutant animals expressing the different transgenes.
(B ) Representative high magnification confocal z-stacks of ORNs innervating the DL4 glomerulus in ten-a null mutant animals expressing Brp-Short-mStraw and mCD8-GFP and stained with antibodies to mStraw (red), GFP (green), and N-Cadherin (blue).
Our previous work revealed that prdx-2-mutant animals express elevated levels of phase 2 detoxification genes, including gcs-1 that encodes a key enzyme in glutathione biosynthesis.
Nevertheless, 30% of prdx-2-mutant animals expressing DAF-28 GFP DAF-28 GFPGFP-positive coelackedtes any only 35% of prdx-2-mutant animals contained more than one coelomocyte with detectable GFP-positivecoelomocytes2and
Hence, to test whether loss of PRDX-2 also increased DAF-16 activity, we examined the localization of GFP in transgenic wild-type and prdx-2-mutant animals expressing DAF-16::GFP (Lin et al., 2001).
Of interest, the C. elegans PHF8 homolog is highly expressed in neurons, and mutant animals show impaired locomotion.
Midbody of wild-type and mutant L4 animals expressing mRFP in TRNs under the mec-7 promoter (jsIs973 ) showing the wild-type and ptrn-1 mutant morphology of PLM and PVM, as well as the ALM soma.
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