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To address the critical question of whether the bacteria in the BAL samples might reflect upper airway contamination of the bronchoscopes used during the procedure, we sampled multiple tissue sites from eight COPD lung explants removed during transplantation (six single and two bilateral transplants).
This finding is based on the observation of increased incidence of malignant tumors at multiple tissue sites in experimental animals of different species.
One consisted of prostate cancer samples isolated by laser capture microdissection generated by Tomlins et al. [ 5] and the other contained expression array data from primary and metastatic tumours from multiple tissue sites generated by Ramaswamy et al. [ 6].
Collection of a database of normal samples from multiple tissue sites will be needed to use ISOpure; however, as shown by our deconvolution of the Director's Challenge cohort of 443 samples, deconvolution can even use existing datasets of normal samples, if the collection protocol and platform are sufficiently similar.
In vivo and in vitro studies have suggested that meats cooked at a high temperature generate heterocyclic amines (HCAs) and other potent mutagens and carcinogens, which can induce tumours in multiple tissue sites in many animal species (Spingarn et al, 1980; Wakabayashi et al, 1992; Okochi et al, 1999; Nagao et al, 2002).
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The special features of D3H2LN subclone are that these cells exhibited more important tumor growth than MDA-MB-231 and that when injected in the left heart ventricle, they induced faster metastasis and more multiple tissues sites [15].
We used pyrosequencing of 16S amplicons to analyze the lung microbiome in two ways: first, using bronchoalveolar lavage (BAL) to sample the distal bronchi and air-spaces; and second, by examining multiple discrete tissue sites in the lungs of six subjects removed at the time of transplantation.
GGT is needed to metabolize glutathionylated xenobiotics in the liver and multiple other tissue sites including the lungs, and this is a simple explanation for its elevation in association with increased exposure to xenobiotics.
Elevated levels of several cytokines/chemokines in plasma are consistent with marked immune activation (a cytokine "storm") which could result in increased viral infection and amplification in multiple cells and tissue sites.
MLVA provided discrimination among the otherwise indistinguishable isolates from different animals and from within each host, with VNTR mutations being detected at multiple dates and tissue sites.
MLVA discriminated among the otherwise indistinguishable isolates from different animals and from isolates collected at different time points within each host, with different VNTR alleles being detected at multiple dates and tissue sites.
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various tissue sites
multiple cell sites
several tissue sites
multiple tissue models
multiple tissue cores
multiple tissue interactions
multiple tissue samples
multiple tissue data
multiple tissue layers
multiple tissue sections
multiple tissue characteristics
multiple tissue compartments
multiple tissue segments
multiple tissue interfaces
multiple tissue lineages
multiple tissue types
multiple tissue specimens
multiple tissue boxes
multiple tissue defects
multiple infection sites
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