Using an HP high performance cluster and a centralised dynamic load balancing approach, the simultaneous analysis of multiple tissue-cores were established.
For statistical analyses, only the highest extent or intensity of Ki-67 staining was considered if multiple evaluable tissue cores were available for the same patient.
Sufficient RNA can be isolated from 3 to 5 tissue cores for multiple QRT-PCR analyses (at least 100 reactions) without the need for RNA amplification.
Multiple (at least two) 2-mm tissue cores were taken from the archived formalin-fixed paraffin-embedded (FFPE) blocks from selected areas of DCIS and adjacent-normal tissue for DNA extraction and bisulfite modification.
Moreover, the evaluation of multiple tissue punches per tumour in this study exceeds the number of cores required for adequate representativity of tumour heterogeneity (Goethals et al, 2006).
TMAs are produced by a method of re-locating tissue from histologic paraffin blocks such that tissue from multiple patients can be studied on the same slide (commonly, three to five tissue cores are extracted from each donor block).
"In multi trauma incidents, maybe due to a blast, you might have to generate multiple tissue types," he said.
These controls provided positive staining in all tissue cores.
Three one-millimeter tissue cores from formalin-fixed paraffin embedded donor blocks were precisely arrayed into a new recipient paraffin block that included tumor specimens and controls, which included multiple cores of normal tissue from 10 different organs.
