Simulations of the kind presented here can be expanded to incorporate additional proteins and multiple tissue compartments to model drug pharmacokinetics and pharmacodynamics.
For example in C. elegans, the most widely studied model organism in this context, multiple tissue-specific transgenic models manifesting pathological phenotypes that faithfully recapitulate many cellular and molecular pathologies of complex neurodegenerative disease processes have been used [ 5, 6].
The model includes multiple tissue layers including the epidermis, dermis, hypodermis, and subcutaneous tissue.
A biophysical model comprised of multiple tissue components was developed and was utilized for the determination of CBV using the visual stimulation data.
Measurement of DAG in obese Zucker rat tissues revealed that 1,2-DAG was elevated in multiple tissues in this model of type 2 diabetes.
Finally, CRISPR/Cas9 has been teamed with induced pluripotent stem (iPS) cells to perform multiple tissue engineering tasks including the creation of disease models or the preparation of donor-specific tissues for transplantation.
Figure 6 demonstrates longitudinal change in risk according to this prediction model in patients who contributed multiple tissue specimens.
The key challenge over the individual component models is that FIRM is built on multiple tissue spaces of different volumes, even varying volumes in some cases.
We studied approximately 4000 normal and cancer samples with multiple tissue types from diverse studies, and using linear and nonlinear regression models identified reliable tissue type-invariant DNAm age signatures.
Despite the use of clinical relevant models of hemorrhagic shock (in particular by Poloujadoff et al. [20]), the experimental models are not able to reproduce exactly the conditions and multiple tissue lesions observed in real polytrauma patients.
