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Exact(6)
Briefly, spinal cord tissues from each mouse were suspended in extraction Buffer A containing 0.2 mM PMSF, 0,15 µM pepstatin A, 20 µM leupeptin, 1 mM sodium orthovanadate, homogenized at the highest setting for 2 min, and centrifuged at 1,000× g for 10 min at 4°C.
Briefly, spinal cord tissues from each mouse were suspended in extraction Buffer A containing 0.2 mM PMSF, 0,15 µM pepstatin A, 20 µM leupeptin, 1 mM sodium orthovanadate, homogenized at the highest setting for 2 min, and centrifuged at 1,000 x g for 10 min at 4°C.
Jurkat cells (2 × 10/0.2 ml per mouse) were suspended in sterile PBS and injected subcutaneously into the right flank of the mice.
U937 cells (2 × 10/0.2 ml per mouse) were suspended in sterile PBS and injected subcutaneously into the right flank of the mice.
To generate the intraperitoneal xenograft model, SGC-7901 cells (1 × 10 per mouse) were suspended in 100 μL of PBS, and injected on day 0 into the abdominal cavity.
Caki-1 cells (2 × 10 cells per mouse) and A549 cells (5 × 10 cells per mouse) were suspended in serum-free DMEM medium and subcutaneously injected into the right flanks of female BALB/c nude mice.
Similar(54)
Briefly, extension of the hindlimbs was observed while the mouse was suspended by its tail.
The mouse was suspended by the tail and extension of each hindlimb observed.
For this test, the mouse was suspended by the end of its tail to a bar 35 cm above the floor [20].
When the mouse is suspended by the tail and lowered so that the vibrissae make contact with a solid object, the head is raised and the fore limbs are extended to grasp the object.
First, the mouse is suspended 10 cm over a table, and the stretch of the forelimbs toward the table is observed and evaluated: normal stretch, 0 points; flexion with a delay (2 sec) and/or incomplete, 1 point; abnormal flexion, 2 point.
Related(20)
rats were suspended
mouse were released
mouse were removed
mouse were processed
mouse were confirmed
mouse were proposed
mouse were considered
mouse were collected
mouse were taken
mouse were recorded
mouse were analyzed
mouse were swabbed
mouse were allowed
mouse were treated
mouse were obtained
mouse were investigated
mouse were harvested
mouse were assessed
mouse were evaluated
mouse were fed
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