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Both eyes in each mouse were assessed.
About 30 cells per mouse were assessed in this study.
The total number of tumors and the tumor weight per mouse were assessed in C3(1 TAG/129 female offspring at 110, 130, 150, 170 days of age.
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In the subsequent 90 seconds, the mouse is assessed for reflexes, limb strength, and self-preservation actions.
Using an 8-point scale, the clinical score for each mouse was assessed starting at age 81 d until age 95 d.
A drifting grating of a pre-selected spatial frequency at 100% contrast appeared, and the mouse was assessed for tracking behavior for a few seconds.
The number of tumours in each mouse was assessed by GFP fluorescence (as described previously [13]) at necropsy followed by histology.
At autopsy, each mouse was assessed for any other abnormalities.
Gene expression of each cell type from each mouse was assessed in triplicate.
(D) Body weights of mice were assessed.
The mice were assessed for systemic side effects of therapy, and tumour sizes were measured daily.
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