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Therefore, 8 h p.i., ex vivo NIRF fluorescence images of the organs and the two xenografts (A431/CCK2R and A431/WT) per mouse were taken in combination with the placement of ROIs on these organs to assess semiquantitative fluorescence intensities.
Five independent tumors (each from a different mouse) were taken from each test group for testing.
10 measurements from each mouse were taken at two minutes intervals then a mean value was determined.
Non-serial sections were evaluated from each mouse (3 mice per group) and at least four fields per mouse were taken at a magnification of ×5000.
Photographs of any one mouse were taken within one week, so that variations in coat appearance due to factors such as weight gain would be minimal.
Five independent tumors (each from a different mouse) were taken from MCF7-FO33 and MCF7-C5 groups for testing.
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Consumers make lots of choices about the computer itself -- how fast, how much storage, how much RAM -- but a mouse is taken for granted as just part of the package.
The manual focus function of the IVIS was used to focus on the surface of the skin prior to the scan, and a white light image of the mouse was taken.
The average number of cells counted in each mouse was taken for statistical comparisons.
A photographic image of the mouse was taken, onto which the pseudocolor image representing the spatial distribution of photon counts was projected.
After the serial imaging, a silhouette image of the mouse was taken under white light to obtain the region of interest (ROI) mask of the ischemic and normal hindlimbs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com