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Superior mesenteric arterial rings from adult male Sprague Dawley rats were suspended in Krebs–Henseleit ring baths.
Study Design: Uterine rings from midpregnant (14-day) and late pregnant (21-day) rats were suspended in organ chambers to record spontaneous contractile activity.
BMDM from Fisher 344 rats were suspended in D10, seeded into 96-well plates at a density of 2×105 per well, and allowed to adhere.
The rats were suspended by their tails using a procedure and equipment very similar to those described by Morey-Holton and Globus (2002) [ 7].
The rats were suspended for 21 consecutive days and were monitored for signs of stress, tail circulatory embarrassment, or skin wounds caused by the suspension system.
Uterine strips from pregnant rats were suspended in standard muscle baths and analyzed for changes in isometric contractions in response to in vitro exposure to commercial PCB mixtures (Aroclors) and their dechlorinated products after microbial degradation.
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Heparinized blood, collected by cardial punction from anaesthetized rats, was suspended in 1 volume PBS (pH 7.4, Invitrogen), applied onto the same volume of Histopaque 1077 (Sigma-Aldrich) and centrifuged (750×g) for 30 min. Mononuclear cells were collected and washed with PBS.
In the first task, the rat was suspended 10 cm over a table, and the forelimb stretch towards the table was observed.
Baited traps were suspended approximately.
Isolated rat aortic rings were suspended in organ chambers for isometric tension recording.
Rat mesenteric arteries were suspended in organ chambers for the measurement of isometric tension during phenylephrine-induced contractions.
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