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The organs of each mouse were removed, treated in 10% formalin, and embedded in paraffin.
Both hind limbs of a mouse were removed directly after cervical dislocation and a single cell suspension w/o erythrocytes was generated as mentioned above.
The large and the small intestine of one mouse were removed, longitudinally sliced and flushed with 0.9% sodium chloride solution to remove faeces and blood.
Some genes (79 for human and 31 for mouse) were removed from our dataset due to ambiguous mapping of their IDs.
Both bullae from each mouse were removed.
Five mammary glands per mouse were removed and fat and muscle were dissected away.
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If no response was evident by 60 sec the mouse was removed to prevent injury.
After suturing, the mouse was removed from the stereotaxic frame and allowed to recover from anesthesia.
The brain of each mouse was removed and mechanically triturated with a fire-polished Pasteur pipette.
Once a sperm plug was found the male mouse was removed and females were housed individually and pregnancy rate in each treatment group recorded.
The mice were anesthetized and approximately 20 30% of the calculated blood volume (∼0.55 ml for a 20 g mouse) was removed.
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