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The femur and tibia from both sides of a C57BL/6 mouse were harvested and flushed into a petri dish.
Thymus, bone marrow (from two femurs per mouse), spleen, and lymph nodes (LN) (two inguinal, axillary, lateral axillary, and mesenteric per mouse) were harvested and dispersed into single-cell suspensions by forcing the tissue through a 70 µm cell strainer (Falcon, Becton Dickinson Labware, Bedford, MA).
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The dorsal skin of each mouse was harvested to permit visualisation of intradermal dye leakage.
When a mouse was harvested, one hemisphere from each mouse brain was frozen on dry-ice and kept at -80 °C, and these tissues were used for ELISA analysis.
The sera and splenocytes of mice were harvested for further analysis.
Furthermore, major organs from the MoS2 ICG-treated MoS2 ICG-treatedted, including the heart, liver, kidney, lung, and spleen.
Study Design: Developmental tissues (embryo, decidua, placenta) from timed pregnant C57B16/J mice were harvested on days 6 though 18.
TA muscles from RNF13 -/- and RNF13 +/+ mice were harvested at different time points after damage (0, 4 h, and 12 h, and 1 d and 3 d).
For ex vivo imaging studies, the whole aortas (n = 9 from ApoE-KO and n = 4 from control mice) were harvested free from adventitial tissue for Sudan IV staining and autoradiography.
Tissues from WT and Xiap deleted mice were harvested and total RNA was isolated.
Lungs of mice were harvested on day 6 after i.n.n
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