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Because of the indication that the intracellular domain of APP is important for axonal transport of APP and cargo molecules, we analyzed the synaptic distribution of neural proteins.
Therefore, to test whether CD4+ T cell help could be influencing the biodistribution of CD8+ T cell via effects on these molecules, we analyzed the expression of gut homing molecules on CFSE-labeled OT-1 T cells in ILN, CLN, MLN and SPL within 3 days after adoptive transfer (Figure 3, left panels).
To further check whether oral administration of abalone visceral extract also affected expression levels of metastasis related molecules, we analyzed the mRNA levels of VEGF, FGF and MMP-13 [ 28, 29].
To further test if suppression of NF-κB signaling also affects the expression of adhesion molecules, we analyzed the expression of VCAM-1, a known NF-κB signaling target, in more detail.
To rule out the possibility that the observed difference in miR-34a level between miR-treated and water treated-control groups was due to spurious amplification of unrelated molecules, we analyzed the RT-qPCR products at the end of the reactions using gel electrophoresis.
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To determine the fluorescence intensity of a single ParAG16V-YFP-His6 (or ParA-YFP) molecule, we analyzed only spots with a fluorescence profile that showed single-step photobleaching.
To verify that the target of PAK1 kinase in this case is also Ser-55 in vimentin molecule we analyzed the effect of expression of Vim(S55E) on MMP.
As mtDNA is a circular molecule, we analyzed the ChIP-seq peaks using two mtDNA references, namely the revised Cambridge Reference Sequence (GenBank number NC_012920) (Andrews et al. 1999) and the same sequence in which nucleotide positions 1 600 were removed and pasted at the end of the sequence.
As human keratinocytes exposed to UV radiation show protective AKT-mediated programs involving NF- κB and the pro-apoptotic BAD molecule, we analyzed the NF- κB and BAD cascades in keratinocyte cultures pre-treated with Ly29 and stimulated with IFN- γ plus TNF- α.
To understand the binding between Dpl and PrP molecules further, we analyzed the kinetics of binding between these proteins using Surface Plasmon Resonance (SPR) biosensor technology.
Furthermore, since TNFα, IL1α, and IL6 are important regulators of endothelial adhesion molecule expression we analyzed expression of ICAM, VCAM, E-selectin, and P-selectin from the tissue (Figure 5C).
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