To obtain a structure for these molecules, we conducted searches in PubChem [22], ChemSpider [23], and Google [24].
In order to select the most relevant set of molecules, we conducted comparisons between the potent PA6 cell line and mouse embryonic fibroblasts (MEF), a mouse kidney cell line MM55K, and subtypes of PA6 and MS5 lines that lack DA-inducing activity.
To determine a critical molecule involved in attraction of THP-1 cells among the NTHI-induced SLF-derived molecules, we conducted migration assays with a viral CC chemokine inhibitor (vCCI).
To estimate the binding free energy contributions of all twenty amino acids at each of the nine peptide positions that interact with the MHC class II molecule, we conducted extensive in silico mutations (see Materials and Methods for details).
In order to examine the effect of drug binding at the active site on the conformation of the attached fluorescein molecule, we conducted molecular modelling on the free enzyme (E) and ES* states of the labelled V216C mutant with penicillin G as the substrate.
DOI: http://dx.doi.org/10.7554/eLife.00654.012 To reveal the fraction of SSTR3 molecules undergoing active transport, we conducted a differential analysis of single SSTR3 molecule movements in cilia of control vs antimycin/deoxyglucose-treated cells.
To determine the effect of co-stimulatory molecules in established arthritis, we conducted the same in vitro experiments by using CTLA-4Ig, anti-ICOS, and anti-CD40L mAbs.
To identify molecules involved in this process, we conducted a forward genetic screen in C. elegans and identified cima-1.
To elucidate potential signaling pathways and adhesion molecules that influence mesoderm migration, we conducted a screen of a library comprising 311 insertions at the presumed 5′ end of genes encoding cell surface or secreted (CSS) factors.
Given the potential for, and growing interest in, EC-derived molecules as biomarkers in sepsis, we conducted a systematic review of the current published literature of biomarkers to determine their performance in predicting the severity of sepsis and clinical outcomes.
To quantitate the depth of the single EGFP molecules that we can image, we conducted the following experiments: we scanned the laser focus within a stained sup-T1 cell; once a single EGFP molecule was identified through recording of stepwise photobleaching, we moved the microfluidic chamber in -z direction (Fig. 1(a)) until the cell boundary hit the laser focus.
